Screen Format Terms
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| + | ====== Screen Format Terms ====== | ||
| + | ^ Term ^ Description ^ | ||
| + | ^ Pool | Cells are screened in a pooled format and the output is detected via deep sequencing of integrated sgRNAs in the DNA pool. | | ||
| + | ^ Array |Cells are screened in an arrayed format requiring high throughput screening technology. May be used to detect phenotypes via automated microscopy or fluorescence/luminescence. | | ||
| + | ^ In vivo | Cells are exposed to an sgRNA library in culture, followed by transplantation into an organism to assess phenotypes in vivo. After a selection phase (e.g., expression of a reporter, outgrowth of a tumor, resistance to therapy), the cells are sequenced to identify enriched and/or depleted sgRNAs . | | ||
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| + | [[:orcs:curation_guide|Back to ORCS Curation Guide]] | ||