Screen Format Terms

Term Description
Pool Cells are screened in a pooled format and the output is detected via deep sequencing of integrated sgRNAs in the DNA pool.
Array Cells are screened in an arrayed format requiring high throughput screening technology. May be used to detect phenotypes via automated microscopy or fluorescence/luminescence.
In vivo Cells are exposed to an sgRNA library in culture, followed by transplantation into an organism to assess phenotypes in vivo. After a selection phase (e.g., expression of a reporter, outgrowth of a tumor, resistance to therapy), the cells are sequenced to identify enriched and/or depleted sgRNAs .

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orcs/controlled_vocabulary4.txt · Last modified: 2019/06/27 10:09 by jenn