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CRISPR Library Types and Names
CRISPR Library Types and Methodologies
Name | Methodology |
CRISPRn | Knockout: Generation of knockout cells or animals with loss-function mutations within the targeted genes. |
CRISPRa | Activation: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression on, thereby activating gene expression. |
CRISPRi | Inhibition: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression off, thereby repressing or inhibiting gene expression. |
CRISPRgbc | Barcode: Barcoded libraries include large numbers of unique barcodes for marking individual cells. The barcodes are passed down to daughter cells and are useful for lineage tracing or for studying the dynamics of heterogeneous tumors. |
CRISPR Library Names and Stats
Name | Size | Addgene IDs |
Human | |
GECKO | 18,080 | |
GECKO v2 | 19,050 | AG cat #:1000000048, 1000000049 |
SAM v1 (Zeomycin) | 23,430 | |
SAM v1 (Puromycin) | 23,430 | |
SAM v2 (bleomycin) | 23,430 | |
CRISPRi-v2 (inhibition) | 18,905 | |
CRISPRa-v2 (activation) | 18,915 | |
Avana | 18,675 | |
Brunello | 19,114 | |
hCRISPRi-v2 | 18,905 | |
hCRISPRa-v2 | 23,430 | |
TKO (Toronto Knockout) v1 | 17,661 | |
Toronto Knockout version 3 (TKOv3) | 18,053 | |
Edit-R crRNA Cell Cycle Regulation library | 169 | |
Extended Knockout Library (EKO)) | 19,084 | |
Human Improved Genome-wide Knockout CRISPR Library | 18,010 | |
H1/H2 library (Liu Human CRISPR Knockout Library) | 18,436 | |
Perturb-seq Guide Barcodes (GBC) | NA | |
CRISPRn (Wang, 2014) | 7,114 | |
CRISPRi (Gilbert,2014) | 15,976 | |
CRISPRa (Gilbert, 2014) | 15,977 | |
Human Activity-Optimized CRISPR Knockout Library/CRISPRn (Wang, 2015) | 18,166 | |
CRISPRn (Deans, 2016) | 18,509 | |
CRISPRn (DeJesus, 2016) | 17,974 | |
CRISPRn Minipool (Goodwin, 2017) | 1,120 | |
Bassik Human CRISPR Knockout Library | 20,533 | |
CRISPRn (Wang, 2017) | 18,663 | |
CRISPRn (Riba, 2017) | 18,671 | |
CRISPRn (Potting, 2018) | 18,703 | |
CRISPRn Targeted Library (Manguso, 2017) | 2,368 | |
CRISPRn Targeted Library (Liao, 2018) | |
CRISPRn Targeted Kinase Domain Library (Tarumoto, 2018) | 482 | |
CRISPRn Targeted Transcription Factor Domain Library (Tarumoto, 2018) | 1,427 | |
Mouse | | |
GECKO v2 (mouse) | 20,611 | |
Mouse improved genome-wide library v2 | 18,424 | |
CRISPRn mouse (Morgens, 2017) | 22,414 | |
Murine lentiviral gRNA library (version 2) | 18,424 | |
Genome-wide Murine Library (Wang, 2017) | 18,987 | |
Murine lentiviral gRNA library (version 1) | 19,149 | |
Brie (mouse) | 19,674 | |
Asagio (mouse) | 20,077 | |
Fly | | |
Oxford Fly | 13,501 | |
CRISPRn (Viswanatha, 2018) ) | 13,651 | |
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