CRISPR Library Types and Names
CRISPR Library Types and Methodologies
| Name | Methodology |
| CRISPRn | Knockout: Generation of knockout cells or animals with loss-function mutations within the targeted genes. |
| CRISPRa | Activation: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression on, thereby activating gene expression. |
| CRISPRi | Inhibition: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression off, thereby repressing or inhibiting gene expression. |
CRISPR Library Names and Stats
| Name | Size | Addgene IDs |
| Human | |
| GECKO | 18,080 | |
| GECKO v2 | 19,050 | AG cat #:1000000048, 1000000049 |
| SAM v1 (Zeomycin) | 23,430 | AG cat #:1000000057 |
| SAM v1 (Puromycin) | 23,430 | AG cat #:1000000075 |
| SAM v2 (bleomycin) | 23,430 | AG cat #:1000000078 |
| CRISPRi-v2 (inhibition) | 18,905 | AG cat #:83969, 1000000090 |
| CRISPRa-v2 (activation) | 18,915 | AG cat #:83978, 1000000091 |
| Avana | 18,675 | |
| Brunello | 19,114 | AG cat #:73179, 73178 |
| hCRISPRi-v2 | 18,905 | |
| hCRISPRa-v2 | 23,430 | |
| TKO (Toronto Knockout) v1 | 17,661 | AG cat #:1000000069 |
| Toronto Knockout version 3 (TKOv3) | 18,053 | AG cat #:90294 |
| Edit-R crRNA Cell Cycle Regulation library | 169 | |
| Extended Knockout Library (EKO)) | 19,084 | |
| Human Improved Genome-wide Knockout CRISPR Library | 18,010 | AG cat #:67989 |
| H1/H2 library (Liu Human CRISPR Knockout Library) | 18,436 | |
| Perturb-seq Guide Barcodes (GBC) | NA | AG cat #:85968 |
| Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)/(Wang, 2014) | 7,114 | |
| CRISPRi (Weissman, 2014) | 15,976 | AG cat #:62217 |
| CRISPRa (Weissman, 2014) | 15,977 | AG cat #:60956 |
| Human Activity-Optimized CRISPR Knockout Library/CRISPRn (Wang, 2015) | 18,166 | AG cat #:1000000100 |
| CRISPRn (Deans, 2016) | 18,509 | |
| CRISPRn (DeJesus, 2016) | 17,974 | |
| CRISPRn Minipool (Goodwin, 2017) | 1,120 | |
| Bassik Human CRISPR Knockout Library | 20,533 | AG cat #:101926 — 101934 |
| CRISPRn (Wang, 2017) | 18,663 | |
| CRISPRn (Riba, 2017) | 18,671 | |
| CRISPRn (Potting, 2018) | 18,703 | |
| CRISPRn Targeted Library (Manguso, 2017) | 2,368 | |
| CRISPRn Targeted Library (Liao, 2018) | Unknown | |
| Human Kinase Domain-Focused CRISPR Knockout Library | 482 | |
| CRISPRn Targeted Transcription Factor Domain Library (Tarumoto, 2018) | 1,427 | |
| Focused Ras Synthetic Lethal Human CRISPR Knockout Library | 132 | AG cat #:92352 |
| Mini-human | Unspecified | AG cat #:130630 |
| Human CRISPR Library v.1.1 | Unspecified | |
| Mouse | |
| GECKO v2 (mouse) | 20,611 | AG cat #:1000000052,1000000053 |
| Mouse improved genome-wide library v2 | 18,424 | AG cat #:67988 |
| Bassik Mouse CRISPR Knockout Library | 22,414 | AG cat #:1000000121 — 1000000130 |
| Murine lentiviral gRNA library (version 2) | 18,424 | AG cat #:67988 |
| Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library | 18,987 | AG cat #:1000000096 |
| Murine lentiviral gRNA library (version 1) | 19,149 | AG cat #:50947 |
| Brie (mouse) | 19,674 | AG cat #:73632, 73633 |
| Asagio (mouse) | 20,077 | |
| Fly | |
| Oxford Fly | 13,501 | AG cat #:64750 |
| CRISPRn (Viswanatha, 2018) ) | 13,651 | |
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