The qualification option allows you to add relevant free-text information, if necessary. For example, it can be used to specify the correct genetic context, such as gene A is synthetic lethal with gene B only when gene C is overexpressed. There are often triple mutants that show a synthetic phenotype only when all three genes are deleted. This can be noted using the text box so that users are not mislead about the interaction. However, some papers will show very complicated genetic interactions that are too difficult to curate so use your own discretion when more than three genes are involved. The qualification box can also be used to add other types of information, e.g. a chemical cross-linker was used in an affinity capture assay. It is important to note that even if mutants or domains of proteins were used, we are still noting the interaction. However, it is not necessary to add details about the domains and mutants, etc. in the qualification text box because otherwise the curation would be too time-consuming.

When adding notes for interactions that have both low and high throughput ( LTP and HTP) experiments associated with them, we need to specify whether the notes apply to the HTP or LTP experiment. Otherwise, a HTP note will also be associated with a LTP one and vice versa. We're supposed to use the following headers in the notes:

• High Throughput: (note)
• Low Throughput: (note)

Similarly, if there are particular notes to be associated with different phenotypes for the same genetic interaction pair, the notes cannot be easily linked to the specific phenotypes at this time. Instead, the notes can be added in the same relative order as the phenotypes, but it is best to specify the phenotype that is being qualified in order to avoid confusion.

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