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--- orcs:definitions [2019/10/04 12:24]
jenn created
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 **Dataset**-a dataset includes all the screens associated with a particular publication or pre-publication document. A single publication may have 1-100s of screens associated with it.
-**Screen**-a set of gene level quantitative scores derived from a single experiment. In ORCS screens are named with a number and PMID in order to differentiate them from any other screens in the same publication. For example, if an author published the quantitative gene level scores from an experiment in which a cell line was screened with an sgRNA library and exposed to a drug and also a separate experiment in which the drug was withheld these screen results would both be curated as 1-PMID1234567 and 2-PMID1234567
+**Screen**-a set of gene level quantitative scores derived from a single experiment. Each screen is assigned a number followed by the PubMed ID (e.g. 1-PMID26627737) to ensure publications with mulitple screens have a unique identifier for each individual screen.
+**Negative Selection Screen**-Screen for identifying genes whose perturbation results in cells not surviving the selection mechanism. Typically, the associated phenotype is “viability” or “resistance” and the mutants are underrepresented/depleted after selection. This fitness screen may be used to determine gene essentiality. May also be referred to as a dropout screen.
+**Positive Selection Screen**-Screen for identifying genes whose perturbation results in cells surviving the selection mechanism, e.g drug exposure. Typically, the associated phenotype is “viability” or “resistance” and the mutants are overrepresented/enriched after selection. This fitness screen may be used to detect drug resistance and identify drug targets. Also known as an enrichment screen.
+**Phenotype Screen**-Screen for identifying genes whose perturbation results in other non-fitness phenotypes, such as altered nuclear size or detection of a selectable marker for measuring activity of a particular pathway.
+**CRISPRn/Knockout Library **-A gRNA library designed to generate knockout cells or animals with loss-function mutations within the targeted genes.
+**CRISPRa/Activation Library **-A gRNA library used in conjunction with a modified Cas enzyme fused to protein domains that turn gene expression on, thereby activating gene expression of target genes.
+**CRISPRi/Inhibtion Library **-A gRNA library used in conjunction with a modified Cas enzyme fused to protein domains that turn gene expression off, thereby repressing or inhibiting gene expression without knocking the gene out.
+**Analytical Method **-The statistical or computational method used by the authors to determine which genes in their screen represent hits. These can range from straight-forward read counts to very complex scoring systems with associated confidence values. For a detailed list of the analytical methods currently in ORCS and the relevant references for these methods go [[orcs:controlled_vocabulary6|here]]

Definitions for commonly used terms

Differences