CRISPR Screen Metadata
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orcs:curation_guide:metadata_terms [2020/06/02 15:20] rose |
orcs:curation_guide:metadata_terms [2022/07/12 13:54] jenn |
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^ Field Name ^ Description ^ | ^ Field Name ^ Description ^ | ||
- | ^ Screen Name |Each screen is assigned a number followed by the PubMed ID (e.g. 1-PMID26627737) to ensure publications with mulitple screens have a unique identfier for each individual screen. | | + | ^ Screen Name |Each screen is assigned a number followed by the PubMed ID (e.g. 1-PMID26627737) to ensure publications with multiple screens have a unique identifier for each individual screen. | |
- | ^ Organism | Controlled vocabulary. | | + | ^ Organism | Controlled vocabulary [[orcs:supported organisms|found here]]. | |
- | ^ Screen Type | [[orcs:controlled_vocabulary|Controlled vocabulary]] based on survey of the data. Can be easily modified to accomodate new screen types that may be developed. | | + | ^ Screen Rationale | A free text field to succinctly describe the purpose of the screen in order to provide additional context for the significance of hits. Common rationales include "Cell essential genes" or "Increased drug resistance" but allow the freedom for added specificity when needed such as "Tumor response to drug treatment". | |
- | ^ Experimental Setup | [[orcs:controlled_vocabulary2|Controlled vocabulary]] based on survey of the data. Can be easily modified to accomodate new screen types that may be developed. | | + | ^ Experimental Setup | [[orcs:controlled_vocabulary2|Controlled vocabulary]] based on survey of the data. Can be easily modified to accommodate new screen types that may be developed. | |
^ Duration | Controlled vocabulary of hours, days or doublings. | | ^ Duration | Controlled vocabulary of hours, days or doublings. | | ||
- | ^ Condition Name/Condition Dosage | If the condition is a drug then add an Ontology ID from ChEBi, if a protein ligand add a UniProt ID. If a toxin or another term with no ID the ID field can be left blank. For the dosage there is a numeric field with an associated controlled vocabulary for units. A list of onotology terms currently in use in ORCS can be [[orcs:conditional_onotology_terms|found here]]. | | + | ^ Condition Name/Condition Dosage | Ontology IDs are added for various conditions: drug/chemical (ChEBI ID), protein ligand (UniProt ID), virus or bacteria (Taxon ID), mutation (relevant gene ID). If a condition does not have an applicable ID, such as a toxin or relevant culture condition the ID field is left blank. Currently if multiple conditions need to be specified for a screen (usually a mutation in addition to another condition) they can be separated using a pipe character. For the dosage there is a numeric field with an associated controlled vocabulary for units. A list of ontology terms currently in use in ORCS can be [[orcs:conditional_onotology_terms|found here]]. | |
^ MOI | Optional (Multiplicity of Infection) should be entered if provided in the publication. | | ^ MOI | Optional (Multiplicity of Infection) should be entered if provided in the publication. | | ||
^ CRISPR Library Name/Type | [[orcs:controlled_vocabulary3|Controlled vocabulary]] terms generated by survey of the literature. Can easily be modified as new libraries are developed. Accession IDs associated when possible.| | ^ CRISPR Library Name/Type | [[orcs:controlled_vocabulary3|Controlled vocabulary]] terms generated by survey of the literature. Can easily be modified as new libraries are developed. Accession IDs associated when possible.| | ||
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^ Phenotype | Ontology terms from APO, CMPO or Controlled Vocabulary as needed. Terms currently in use in ORCS can be [[orcs:phenotype_terms|found here]]. | | ^ Phenotype | Ontology terms from APO, CMPO or Controlled Vocabulary as needed. Terms currently in use in ORCS can be [[orcs:phenotype_terms|found here]]. | | ||
^ Analysis Method | [[orcs:controlled_vocabulary6|Controlled vocabulary]] terms generated by survey of the literature. Can easily be modified as new analysis methods are developed. | | ^ Analysis Method | [[orcs:controlled_vocabulary6|Controlled vocabulary]] terms generated by survey of the literature. Can easily be modified as new analysis methods are developed. | | ||
- | ^ Significance Threshold |If provided by the authors a numeric significance threshold can be applied to any score column entered for a screen. Alternatively if only a list of hits was published the curator can mark the screen data as "all significant" or if a yes/no hit vaue was used the author can specify a boolean column. | | + | ^ Significance Threshold |When a significance threshold is given in a paper, it can be applied to any score column for a screen and even to multiple score columns based on the analysis method. If only a list of significant hits was published, then all of the gene hits are uploaded as “all significant”. Alternatively, if a yes/no hit value was used to identify gene hits in a paper, then the genes can be uploaded by specifying they're hit status using a boolean column. | |
[[:orcs:curation_guide|Back to ORCS Curation Guide]] | [[:orcs:curation_guide|Back to ORCS Curation Guide]] |