Enzyme Terms
Differences
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orcs:controlled_vocabulary5 [2019/06/27 10:31] jenn |
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| ^ CPF1 | Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Cpf1 cleaves DNA via a staggered DNA double-stranded break.| | ^ CPF1 | Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Cpf1 cleaves DNA via a staggered DNA double-stranded break.| | ||
| ^ CAS13a | Class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector which can be engineered for mammalian cell RNA knockdown and binding. | | ^ CAS13a | Class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector which can be engineered for mammalian cell RNA knockdown and binding. | | ||
| + | ^ AncBE4max | Cytidine base editor that converts target C:G base pairs to T:A, introducing point mutations instead of double strand breaks at target. These point mutations can be used to induce gene KO by introducing premature termination codons or splicing disruptions. AncBE4max has a bis-bpNLS BE4 with the Anc689 APOBEC and GenScript codons. | | ||
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| [[:orcs:curation_guide|Back to ORCS Curation Guide]] | [[:orcs:curation_guide|Back to ORCS Curation Guide]] | ||