Enzyme Terms
Differences
This shows you the differences between two versions of the page.
| Next revision | Previous revision Last revision Both sides next revision | ||
|
orcs:controlled_vocabulary5 [2019/06/27 10:31] jenn created |
orcs:controlled_vocabulary5 [2022/07/25 09:43] jenn |
||
|---|---|---|---|
| Line 2: | Line 2: | ||
| - | ^ Term ^ Description ^ | + | ^ Term ^ Description ^ |
| ^ CAS9 |CRISPR-associated protein 9 (Cas9) nuclease. To initiate programmed DNA targeting, Cas9 binds both crRNA and tracrRNA in an RNA-duplex. The first 20 nucleotides of the crRNA guide Cas9 to cleave any complementary genomic sequence located next to a short protospacer adjacent motif (PAM). A convenient fusion of the crRNA and tracrRNA produces a chimeric single guide RNA (sgRNA) that is sufficient to target Cas9 as well. Cas9 isolated from the bacteria Streptococcus pyogenes (SpCas9) is most commonly used in CRISPR screens currently and the CAS9 variants discussed below should be assumed to be SpCas9 unless otherwise noted. (PMID: 27248712) | | ^ CAS9 |CRISPR-associated protein 9 (Cas9) nuclease. To initiate programmed DNA targeting, Cas9 binds both crRNA and tracrRNA in an RNA-duplex. The first 20 nucleotides of the crRNA guide Cas9 to cleave any complementary genomic sequence located next to a short protospacer adjacent motif (PAM). A convenient fusion of the crRNA and tracrRNA produces a chimeric single guide RNA (sgRNA) that is sufficient to target Cas9 as well. Cas9 isolated from the bacteria Streptococcus pyogenes (SpCas9) is most commonly used in CRISPR screens currently and the CAS9 variants discussed below should be assumed to be SpCas9 unless otherwise noted. (PMID: 27248712) | | ||
| ^ dCAS9 | A nuclease ‘dead’ Cas9 (dCas9) variant, this is able to bind DNA in a sequence specific manner but not cleave it.| | ^ dCAS9 | A nuclease ‘dead’ Cas9 (dCas9) variant, this is able to bind DNA in a sequence specific manner but not cleave it.| | ||
| Line 11: | Line 11: | ||
| ^ CPF1 | Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Cpf1 cleaves DNA via a staggered DNA double-stranded break.| | ^ CPF1 | Cpf1 is a single RNA-guided endonuclease lacking tracrRNA, and it utilizes a T-rich protospacer-adjacent motif. Cpf1 cleaves DNA via a staggered DNA double-stranded break.| | ||
| ^ CAS13a | Class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector which can be engineered for mammalian cell RNA knockdown and binding. | | ^ CAS13a | Class 2 type VI RNA-guided RNA-targeting CRISPR–Cas effector which can be engineered for mammalian cell RNA knockdown and binding. | | ||
| + | ^ AncBE4max | Cytidine base editor enzyme that converts target C:G base pairs to T:A, introducing point mutations instead of double strand breaks at target. These point mutations can be used to induce gene KO by introducing premature termination codons or splicing disruptions. AncBE4max has a bis-bpNLS BE4 with the Anc689 APOBEC and GenScript codons. | | ||
| + | |||
| [[:orcs:curation_guide|Back to ORCS Curation Guide]] | [[:orcs:curation_guide|Back to ORCS Curation Guide]] | ||