Enzyme Terms
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orcs:controlled_vocabulary5 [2019/06/27 10:31] jenn created |
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| - | ^ Term ^ Description ^ | + | ^ Term ^ Description ^ |
| ^ CAS9 |CRISPR-associated protein 9 (Cas9) nuclease. To initiate programmed DNA targeting, Cas9 binds both crRNA and tracrRNA in an RNA-duplex. The first 20 nucleotides of the crRNA guide Cas9 to cleave any complementary genomic sequence located next to a short protospacer adjacent motif (PAM). A convenient fusion of the crRNA and tracrRNA produces a chimeric single guide RNA (sgRNA) that is sufficient to target Cas9 as well. Cas9 isolated from the bacteria Streptococcus pyogenes (SpCas9) is most commonly used in CRISPR screens currently and the CAS9 variants discussed below should be assumed to be SpCas9 unless otherwise noted. (PMID: 27248712) | | ^ CAS9 |CRISPR-associated protein 9 (Cas9) nuclease. To initiate programmed DNA targeting, Cas9 binds both crRNA and tracrRNA in an RNA-duplex. The first 20 nucleotides of the crRNA guide Cas9 to cleave any complementary genomic sequence located next to a short protospacer adjacent motif (PAM). A convenient fusion of the crRNA and tracrRNA produces a chimeric single guide RNA (sgRNA) that is sufficient to target Cas9 as well. Cas9 isolated from the bacteria Streptococcus pyogenes (SpCas9) is most commonly used in CRISPR screens currently and the CAS9 variants discussed below should be assumed to be SpCas9 unless otherwise noted. (PMID: 27248712) | | ||
| ^ dCAS9 | A nuclease ‘dead’ Cas9 (dCas9) variant, this is able to bind DNA in a sequence specific manner but not cleave it.| | ^ dCAS9 | A nuclease ‘dead’ Cas9 (dCas9) variant, this is able to bind DNA in a sequence specific manner but not cleave it.| | ||