BioGRID Curation Guide
Differences
This shows you the differences between two versions of the page.
| Both sides previous revision Previous revision | Next revision Both sides next revision | ||
|
curation_guide [2013/11/06 19:24] jenn [Curation Questions and Answers] |
curation_guide [2013/11/07 11:32] jenn [Curation Questions and Answers] |
||
|---|---|---|---|
| Line 35: | Line 35: | ||
| - **When is a protein considered to interact with itself as a bait and hit?** | - **When is a protein considered to interact with itself as a bait and hit?** | ||
| * This type of interaction is reserved for situations where clear evidence has been shown for the presence of a dimer or multimer. The most common experiment involves tagging a single protein with two different tags, pulling down with one and then blotting for the other. "Affinity Capture-Western" applies if it's an //in vivo// experiment and "Reconstituted Complex" if it's an //in vitro// one. Crystal structures may also show dimerization. | * This type of interaction is reserved for situations where clear evidence has been shown for the presence of a dimer or multimer. The most common experiment involves tagging a single protein with two different tags, pulling down with one and then blotting for the other. "Affinity Capture-Western" applies if it's an //in vivo// experiment and "Reconstituted Complex" if it's an //in vitro// one. Crystal structures may also show dimerization. | ||
| + | - **If I have to arbitrarily choose a directionality for an interaction should I enter the interaction twice (once in each direction)?** | ||
| + | *No, we curate using a spoke model and entering interactions in both directions will artificially inflate the number of interactions in the database (making it appear as if an interaction has been shown twice in a paper when it has not). Choose the directionality that makes the most sense based on BioGRID guidelines in the Directionality of Interactions (Baits/Hits) section and only enter the interaction once. | ||
| - **What if I'm not sure how to curate a paper?** | - **What if I'm not sure how to curate a paper?** | ||
| * If you are having trouble curating a difficult paper, then it is a good idea to email other curators for advice. | * If you are having trouble curating a difficult paper, then it is a good idea to email other curators for advice. | ||