For papers in which curation of the screen results is not possible from published data, including supplemental tables and on-line resources, curators will attempt to contact the authors directly to request more information. This includes instances in which no quantitative scores have been published or score thresholds are unable to be determined after close review.
Curators will use the contact information provided for the publication for both the corresponding author and when possible the first author as well.
In order to increase the availability of CRISPR screen data to the scientific community curators will reach out at least twice to request the data. If there is no response after two contact attempts authors may mark the dataset as “unable to attain”.
For authors who do respond curators should request a full set of quantitative values if needed and the following metadata for each screen:
PubMed ID:
Screen name:
Supplementary File: Name of file (specify sheet if Excel file with multiple sheets)
Scores Size: # of genes with scores provided in the file
Number of Hits: # of hits in CRISPR screen
Full Dataset Size: # of genes in CRISPR screen
Full Dataset Available: Yes/No
Analysis Method: e.g. MaGeCK or RSA
Screen Type: Negative Selection/Positive Selection/Phenotype Screen
Screen Format: Pool/Array
Cell Line:
Cell Type:
Species:
Throughput: High Throughput
Enzyme: CAS9 or other?
Phenotype: viability or other?
Experimental Setup: Timecourse or other?
Duration: # Days/Weeks or other?
Condition Name: if applicable
Condition Dosage: if applicable
MOI:
Library: e.g. TKO (Toronto Knockout) v1
Library Type: CRISPRn/CRISPRa/CRISPRi
Library Methodology: Knockout/Activation/Inhibition
Significance: score cutoff, e.g. p-value < 0.05