CRISPR Library Types and Names
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orcs:controlled_vocabulary3 [2019/10/02 10:52] jenn created |
orcs:controlled_vocabulary3 [2019/10/14 15:00] rose [CRISPR Library Types and Methodologies] |
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| ^ CRISPRa |Activation: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression on, thereby activating gene expression. | | ^ CRISPRa |Activation: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression on, thereby activating gene expression. | | ||
| ^ CRISPRi |Inhibition: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression off, thereby repressing or inhibiting gene expression. | | ^ CRISPRi |Inhibition: Use of CRISPR with a modified Cas enzyme fused to protein domains that turn gene expression off, thereby repressing or inhibiting gene expression. | | ||
| - | ^ CRISPRgbc |Barcode: Barcoded libraries include large numbers of unique barcodes for marking individual cells. The barcodes are passed down to daughter cells and are useful for lineage tracing or for studying the dynamics of heterogeneous tumors. | | + | |
| ===== CRISPR Library Names and Stats ===== | ===== CRISPR Library Names and Stats ===== | ||
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| ^ GECKO |18,080| | ^ GECKO |18,080| | ||
| ^ GECKO v2 |19,050|AG cat #:1000000048, 1000000049| | ^ GECKO v2 |19,050|AG cat #:1000000048, 1000000049| | ||
| - | ^ SAM v1 (Zeomycin) |23,430|AG cat #:1000000057 | + | ^ SAM v1 (Zeomycin) |23,430|AG cat #:1000000057| |
| - | ^ SAM v1 (Puromycin) |23,430|AG cat #:1000000075 | + | ^ SAM v1 (Puromycin) |23,430|AG cat #:1000000075| |
| - | ^ SAM v2 (bleomycin) |23,430|AG cat #:1000000078 | + | ^ SAM v2 (bleomycin) |23,430|AG cat #:1000000078| |
| - | ^ CRISPRi-v2 (inhibition)|18,905|AG cat #:83969, 1000000090 | + | ^ CRISPRi-v2 (inhibition)|18,905|AG cat #:83969, 1000000090| |
| - | ^ CRISPRa-v2 (activation)|18,915| | + | ^ CRISPRa-v2 (activation)|18,915|AG cat #:83978, 1000000091| |
| ^ Avana |18,675| | ^ Avana |18,675| | ||
| - | ^ Brunello |19,114| | + | ^ Brunello |19,114|AG cat #:73179, 73178| |
| ^ hCRISPRi-v2 |18,905| | ^ hCRISPRi-v2 |18,905| | ||
| ^ hCRISPRa-v2 |23,430| | ^ hCRISPRa-v2 |23,430| | ||
| - | ^ TKO (Toronto Knockout) v1|17,661|AG cat #:1000000069 | + | ^ TKO (Toronto Knockout) v1|17,661|AG cat #:1000000069| |
| - | ^ Toronto Knockout version 3 (TKOv3)|18,053| | + | ^ Toronto Knockout version 3 (TKOv3)|18,053|AG cat #:90294| |
| ^ Edit-R crRNA Cell Cycle Regulation library|169| | ^ Edit-R crRNA Cell Cycle Regulation library|169| | ||
| ^ Extended Knockout Library (EKO))|19,084| | ^ Extended Knockout Library (EKO))|19,084| | ||
| - | ^ Human Improved Genome-wide Knockout CRISPR Library|18,010| | + | ^ Human Improved Genome-wide Knockout CRISPR Library|18,010|AG cat #:67989| |
| ^ H1/H2 library (Liu Human CRISPR Knockout Library)|18,436| | ^ H1/H2 library (Liu Human CRISPR Knockout Library)|18,436| | ||
| - | ^ Perturb-seq Guide Barcodes (GBC)|NA| | + | ^ Perturb-seq Guide Barcodes (GBC)|NA|AG cat #:85968| |
| - | ^ CRISPRn (Wang, 2014)|7,114| | + | ^ Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)/(Wang, 2014)|7,114|AG cat #:51043-51048 |
| - | ^ CRISPRi (Gilbert,2014)|15,976| | + | ^ CRISPRi (Weissman, 2014)|15,976|AG cat #:62217| |
| - | ^ CRISPRa (Gilbert, 2014)|15,977| | + | ^ CRISPRa (Weissman, 2014)|15,977|AG cat #:60956| |
| - | ^ Human Activity-Optimized CRISPR Knockout Library/CRISPRn (Wang, 2015)|18,166| | + | ^ Human Activity-Optimized CRISPR Knockout Library/CRISPRn (Wang, 2015)|18,166|AG cat #:1000000100| |
| ^ CRISPRn (Deans, 2016)|18,509| | ^ CRISPRn (Deans, 2016)|18,509| | ||
| ^ CRISPRn (DeJesus, 2016)|17,974| | ^ CRISPRn (DeJesus, 2016)|17,974| | ||
| ^ CRISPRn Minipool (Goodwin, 2017)|1,120| | ^ CRISPRn Minipool (Goodwin, 2017)|1,120| | ||
| - | ^ Bassik Human CRISPR Knockout Library|20,533| | + | ^ Bassik Human CRISPR Knockout Library|20,533|AG cat #:101926 — 101934| |
| ^ CRISPRn (Wang, 2017)|18,663| | ^ CRISPRn (Wang, 2017)|18,663| | ||
| ^ CRISPRn (Riba, 2017)|18,671| | ^ CRISPRn (Riba, 2017)|18,671| | ||
| ^ CRISPRn (Potting, 2018)|18,703| | ^ CRISPRn (Potting, 2018)|18,703| | ||
| ^ CRISPRn Targeted Library (Manguso, 2017)|2,368| | ^ CRISPRn Targeted Library (Manguso, 2017)|2,368| | ||
| - | ^ CRISPRn Targeted Library (Liao, 2018)|| | + | ^ CRISPRn Targeted Library (Liao, 2018)|Unknown| |
| - | ^ CRISPRn Targeted Kinase Domain Library (Tarumoto, 2018)|482| | + | ^ Human Kinase Domain-Focused CRISPR Knockout Library|482|AG cat #:117725 |
| ^ CRISPRn Targeted Transcription Factor Domain Library (Tarumoto, 2018)|1,427| | ^ CRISPRn Targeted Transcription Factor Domain Library (Tarumoto, 2018)|1,427| | ||
| - | ^ Mouse ^ ^ | + | ^ Focused Ras Synthetic Lethal Human CRISPR Knockout Library|132|AG cat #:92352| |
| - | ^ GECKO v2 (mouse) |20,611| | + | ^ Mini-human|Unspecified|AG cat #:130630| |
| - | ^ Mouse improved genome-wide library v2 |18,424| | + | ^ Human CRISPR Library v.1.1|Unspecified| |
| - | ^ CRISPRn mouse (Morgens, 2017) |22,414| | + | ^ Mouse ^ ^^ |
| - | ^ Murine lentiviral gRNA library (version 2) |18,424| | + | ^ GECKO v2 (mouse) |20,611|AG cat #:1000000052,1000000053| |
| - | ^ Genome-wide Murine Library (Wang, 2017) |18,987| | + | ^ Mouse improved genome-wide library v2 |18,424|AG cat #:67988| |
| - | ^ Murine lentiviral gRNA library (version 1)|19,149| | + | ^ Bassik Mouse CRISPR Knockout Library |22,414|AG cat #:1000000121 — 1000000130| |
| - | ^ Brie (mouse)|19,674| | + | ^ Murine lentiviral gRNA library (version 2) |18,424|AG cat #:67988| |
| + | ^ Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library |18,987|AG cat #:1000000096| | ||
| + | ^ Murine lentiviral gRNA library (version 1)|19,149|AG cat #:50947| | ||
| + | ^ Brie (mouse)|19,674|AG cat #:73632, 73633| | ||
| ^ Asagio (mouse) |20,077| | ^ Asagio (mouse) |20,077| | ||
| - | ^ Fly ^ ^ | + | ^ Fly ^ ^^ |
| - | ^ Oxford Fly|13,501| | + | ^ Oxford Fly|13,501|AG cat #:64750| |
| ^ CRISPRn (Viswanatha, 2018) )|13,651| | ^ CRISPRn (Viswanatha, 2018) )|13,651| | ||
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